Effect of refractive index mismatch on forward-to-backward ratios in SHG imaging

verfasst von

Jarno N. Van Der Kolk, Stéphane Bancelin, Charalambos Kioulos, Antonino Calà Lesina, François Légaré, Lora Ramunno

Abstract

Nonlinear optical imaging in the epi-direction is used to image subresolution features. We find that a refractive index mismatch between the object to be imaged and the background medium can change the far-field intensity image. As an example, we study second harmonic generation (SHG) microscopy where the forward-to-backward (F/B) ratio is used to quantify subresolution features. We show both theoretically and experimentally that the inhomogeneous refractive index in collagen tendon tissue creates near-field effects, which can change the F/B ratio by ∼20%-25%, even though the effect is negligible for most of the individual fibrils in the tissue. This is caused by the sensitivity of the backward signal on phase matching conditions.

Externe Organisation(en)

University of Ottawa
Institut national de la recherche scientifique (INRS)

Typ

Artikel

Journal

Optics letters

Band

43

Seiten

5082-5085

Anzahl der Seiten

4

ISSN

0146-9592

Publikationsdatum

15.10.2018

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Atom- und Molekularphysik sowie Optik

Elektronische Version(en)

https://doi.org/10.1364/ol.43.005082 (Zugang: Geschlossen)