Research Focus: Biophotonics | Life Sciences | Environmental Analytics

Cytochrome-c - a protein to see into bacteria.
Raman spectrum of a Nitrosomonas bacterium.

Experimental Methods and Data Analysis

For this, we are working on new or improved experimental and data analysis methods:

  • Multivariate and cluster analysis: for the discrimination and grouping of spectra
  • Raman Resonance Profiles: high-resolution resonance profiles, molecular identfication, and tunable Rayleigh-filters
  • Simulation and image processing: Raman spectra, OCT, opto acoustics, photophysics and -chemistry


  • RRS-OA
    Leaders: M. Wollweber
    Year: 2011
    Sponsors: DFG
  • OCT
    Leaders: B. Roth, M. Rahlves
    Year: 2011
    Year: 2011
  • Research Initiative "Water and Security"
    Leaders: B. Roth, M. Wollweber
    Team: A.-K. Kniggendorf
    Year: 2011
  • Hautkrebsscreening (HKS)
    Entwicklung eines digitalen Dermatoskopiegerätes mit erweitertem Diagnoseumfang für das automatisierte Ganzkörper-Hautkrebs-Screening (Melanom-früherkennung)
    Leaders: M. Wollweber
    Year: 2012
  • Determination of melanoma thickness via optoacoustics and optical coherence tomography (OCT)
    Development of a method for the preoperative determination of the thickness of melanoma suspicious skin lesions.
    Leaders: B. Roth, M. Wollweber, U. Morgner, M. Rahlves
    Year: 2013
    Sponsors: BMBF
  • OPTIMUS -Raman spectroscopy of microplastics, microbes, and trace elements
    online detection of contaminated microplastics in streaming drinking water with Raman spectroscopy for the BMBF joint project OPTIMUS.
    Leaders: B. Roth
    Team: A.-K. Kniggendorf
    Year: 2016
    Sponsors: Bundesministerium für Bildung und Forschung (BMBF)
  • Smartphone based optical point of care tests
    Leaders: K. Bremer, B. Roth
    Team: M. Rahlves
    Year: 2017
    Sponsors: Bundesministerium für Wirtschaft und Energie (BMWi) und Europäischer Sozialfond (ESF)
  • AMIRA - Analysis of microbial relations in vivo using Raman microscopy
    Projekt AMIRA is a cooperation between HOT and the department for Hydrobiology and Environmental Biotechnology at the Institute for Sanitary Engineering and Waste Management (ISAH) of the LUH and intends to establish cytochrome c-resonant Raman microscopy (CRRM) for the continuous analysis of bacterial relations in native, undisturbed biofilms in vivo and in situ with a resolution at cell level. This will allow for the time the direct observation of ongoing biofilm formation including phylogenetic information and phenotypic adaptation in complex biofilms in vivo.
    Leaders: A.-K. Kniggendorf
    Year: 2018
    Sponsors: Deutsche Forschungsgemeinschaft (DFG) - 397827619


Dr. Ann-Kathrin Kniggendorf
Dr. Ann-Kathrin Kniggendorf