Biophotonics & Analytics: Confocal Raman Microscopy

Raman chemical imaging identifying colonies of different bacteria within a living biofilm.
Confocal Raman microscope CRM 200.

Confocal Raman microscopy combines the properties of confocal microscopy - high, spatial resolution in 3D - with the advantages of Raman spectroscopy - precise substance identification via the vibrational modes of the involved molecules.

The HOT uses confocal Raman microscopy for example to

  • non-invasively analyze living biofilms
  • non-invasively identify bacteria species
  • detection of microplastics in complex environmental samples
  • functional analysis of proteins, peptides, and amino acids

often using specific molecular resonances for signal enhancing or directly in the form of resonance Raman spectroscopy.

See also

  • Thomas Dieing, Olaf Hollricher, Jan Toporski (Eds), Confocal Raman Microscopy, Springer 2010,
    ISBN 978-3-642-12521-8, e-ISBN 978-3-642-12522-5
  • Arnaud Zoubir (Edt.), Raman Imaging, Springer 2012,
    ISBN 978-3-642-28251-5, e-ISBN 978-3-642-28252-2
  • Pavel Matousek, Michael D. Morris (Eds.), Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields, Springer 2010,
    ISBN 978-3-642-02648-5, e-ISBN 978-3-642-02649-2



Dr. Ann-Kathrin Kniggendorf
Dr. Ann-Kathrin Kniggendorf