Monte Carlo simulation of the influence of internal optical absorption on the external Raman signal for biological samples
Abstract
Proper understanding of Raman spectroscopic signals from biological samples requires the quantification of internal signal absorption and its effect on the Raman spectra detected outside the samples under study. In this paper, we describe an efficient Monte Carlo method to simulate Raman scattering in biological tissues and solutions and compare the findings with experimental results obtained in samples with different absorber concentrations and optical properties. As an illustrative example, we focus on solutions of beta-carotene (bCar) in ethanol with different concentrations of absorber (ink) added. We find good agreement between simulation and experiment, thus indicating a way to quantify the influence of internal signal absorption in Raman measurements.
Details
- Organisation(s)
-
Hannover Centre for Optical Technologies (HOT)
- External Organisation(s)
-
Immanuel Kant Baltic Federal University
Amur State University
- Type
- Article
- Journal
- Journal of the Optical Society of America A: Optics and Image Science, and Vision
- Volume
- 36
- Pages
- 877-882
- No. of pages
- 6
- ISSN
- 1084-7529
- Publication date
- 05.2019
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials, Atomic and Molecular Physics, and Optics, Computer Vision and Pattern Recognition
- Electronic version(s)
-
https://doi.org/10.1364/JOSAA.36.000877 (Access:
Closed
)
